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KMID : 1144820190250040321
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대한의생명과학회지
2019 Volume.25 No. 4 p.321 ~ p.330
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Prevalence and Diversity of MBL Gene-Containing Integrons in Metallo-β-Lactamase (MBL)-Producing Pseudomonas spp. Isolates Disseminated in a Korean Hospital
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Yum Jong-Hwa
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Abstract
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Carbapenem is recently considered as the last resort of the therapeutics for gram negative bacterial infection. Increasing of organisms producing metallo-β-lactamase (MBL), we have difficulty in choosing the antimicrobial agents. Among 345 clinical isolates of Pseudomonas spp., 61 isolates (17.7%) were positive for the modified imipenem or meropenem-Hodge test and 55 isolates (15.9%) were positive for the imipenem-EDTA + SMA double disk synergy test (DDS). PCR and sequencing of blaVIM-2-allele and blaIMP-1-allele showed that 17 isolates of Pseudomonas aeruginosa, 9 isolates of Pseudomonas taiwnensis and 2 Pseudomonas plecoglossicida had blaVIM-2, and 22 isolates of P. aeruginosa and one Pseudomonas otitidis had blaIMP-6. These MBL genes were all in class 1 integron. The size of class 1 integron with blaVIM-2 ranged from 3.5 kb to 5.5 kb in clinical isolates of Pseudomonas spp. including P. aeruginosa. blaVIM-2 was most of ten located first in the class 1 integron, sometimes in the second or third position, and these integrons of ten had aacA4 or aadA1. Strict infection control measures are needed to more effectively prevent further spread of these MBL-producing Pseudomonas spp. In addition, MBL-producing Pseudomonas spp. is expected to continue to spread in various countries and regions.
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KEYWORD
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Pseudomonas spp., Metallo-β-lactamase, MBL, blaVIM-2, blaIMP-6, Carbapenem resistant
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